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Cotransport of proline and Li + in Escherichia coli
Author(s) -
Tsuchiya Tomofusa,
Yamane Yoshiko,
Shiota Sumiko,
Kawasaki Takashi
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80272-0
Subject(s) - escherichia coli , cotransporter , chemistry , proline , biochemistry , sodium , amino acid , organic chemistry , gene
Uptake of Li + induced by the addition of proline to a cell suspension of Escherichia coli was detected using an Li + ‐selective electrode. This Li + uptake was inhibited by L‐azetidine 2‐carboxylic acid, a competitive inhibitor of the proline transport system. Thus, direct evidence for Li + ‐proline cotransport via the proline transport system was obtained. Kinetic parameters of the Li + uptake were determined.