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ATP production coupled to the denitrification of nitrate in Rhizobium japonicum , grown in cultures and in bacteroids from Glycine max
Author(s) -
Bhandari Basant,
Naik M.S.,
Nicholas D.J.D.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80271-9
Subject(s) - nitrate , denitrification , glycine , bradyrhizobium japonicum , chemistry , biochemistry , rhizobium , bacteria , biology , nitrogen , rhizobiaceae , amino acid , organic chemistry , symbiosis , gene , genetics
The denitrification of nitrate in membrane fractions of Rhizobium japonicum CC705 grown in culture solutions with nitrate and those from bacteroids of Glycine max was coupled to ATP production. The ATP/2e − ratios for electron transfer from succinate and NADH to NO − 3 and O 2 in membrane fractions of R. japonicum grown with nitrate were: succinate to O 2 , 2.0; succinate to nitrate, 1; NADH to O 2 , 3; NADH to nitrate, 2. In membrane fractions of bacteroids the value for succinate to nitrate was also 1.