Premium
Functional groups on ‘Z’ DNA recognized by monoclonal antibodies
Author(s) -
Lee Jeremy S.,
Woodsworth Mary L.,
Latimer Laura J.P.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80267-7
Subject(s) - monoclonal antibody , dna , chemistry , antibody , microbiology and biotechnology , computational biology , biology , genetics , biochemistry
Both brominated poly[d(GC)] and poly[d(Gm 5 C)] form stable left‐handed Z‐DNA structures at physiological ionic strengths. These two antigens were use to prepare monoclonal antibodies from immunized mice. The specificity of the antibodies was studied in detail with a solid‐phase radioimmune assay as well as by means of competition experiments. Both immunogens produced several relatively non‐specific antibodies but two types of very specific antibody were also distinguished. The first binds poly[d(Gm 5 C)] but not brominated poly[d(GC)] while the other has the opposite specificity and will only bind the brominated polymer.