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Proinsulin‐like material in mouse foetal brain cell cultures
Author(s) -
Birch Nigel P.,
Christie David L.,
Renwick Alistair G.C.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80266-5
Subject(s) - proinsulin , trypsin , insulin , size exclusion chromatography , endocrinology , medicine , chemistry , chromatography , biochemistry , biology , enzyme
Two main forms of immunoreactive insulin have been identified in cultures of foetal mouse brain using HPLC and gel filtration. The major component which resembled proinsulin was converted by trypsin to the minor form which was similar to authentic pancreatic insulin in chromatographic behaviour. Both components showed immunological properties comparable to insulin and proinsulin including sensitivity of the former to reduction and alkylation.