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β‐Hydroxyaspartic acid in vitamin K‐dependent plasma proteins from scorbutic and warfarin‐treated guinea pigs
Author(s) -
Stenflo Johan,
Fernlund Per
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80264-1
Subject(s) - hydroxylation , aspartic acid , chemistry , vitamin , proline , ascorbic acid , guinea pig , amino acid , biochemistry , vitamin c , citric acid , medicine , endocrinology , biology , enzyme , food science
β‐Hydroxyaspartic acid is a rare amino acid, present in all vitamin K‐dependent plasma proteins except prothrombin, and is formed by a post‐translational hydroxylation of aspartic acid. We have now investigated whether this hydroxylation, like that of proline in collagen, is vitamin C‐dependent. The vitamin K‐dependent plasma proteins were isolated from normal and scorbutic guinea pig plasma by barium citrate adsorption and the β‐hydroxyaspartic acid content was determined. Compared with normal animals, scorbutic animals showed no significant reduction of β‐hydroxyaspartic acid content. In warfarin‐treated animals there was a decreased content of both β‐hydroxyaspartic acid and γ‐carboxyglutamic acid in the barium citrate adsorbed fraction. It was concluded that the post‐translational hydroxylation of aspartic acid is unlikely to be vitamin C‐dependent.