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Effect of the DNA‐binding domain of poly(ADP‐ribose)synthetase on accurate transcription initiation in a HeLa cell lysate
Author(s) -
Ohtsuki Masahiko,
Sekimizu Kazuhisa,
Agemori Michiko,
Shizuta Yutaka,
Natori Shunji
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80261-6
Subject(s) - transcription (linguistics) , hela , microbiology and biotechnology , lysis , dna , dna binding domain , enzyme , chemistry , poly adp ribose polymerase , biochemistry , papain , ribose , cell , transcription factor , biology , polymerase , gene , philosophy , linguistics
The effect of papain‐digested fragments of poly(ADP‐ribose)synthetase on accurate transcription initiation by RNA polymerase II was studied using a HeLa cell lysate. It was found that the DNA‐binding domain of the enzyme, like the intact enzyme, preferentially suppressed random transcription initiation, whereas the automodification domain had no effect on transcription.