Premium
Cell‐free synthesis of putative precursors for envelope membrane polypeptides of spinach chloroplasts
Author(s) -
Flügge U.I.,
Wessel D.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80257-4
Subject(s) - spinach , chloroplast membrane , biochemistry , membrane , methionine , chloroplast , membrane protein , immunoprecipitation , cell envelope , chemistry , protein biosynthesis , biology , amino acid , thylakoid , gene , escherichia coli
Protein synthesis in the presence of [ 35 S]methionine was carried out in a wheat germ cell‐free system programmed with spinach leaf poly(A) + RNA. After translation, identification of the putative precursors of the envelope membrane polypeptides was performed by immonoprecipitation using antibodies against 3 envelope membrane polypeptides; M r 22 000, 29 000 (phosphate translocator) and 36 000. The identities of the immunoprecipitated precursors are established by performing competition studies. The results show that the precursor of the M r 22 000 envelope membrane polypeptide is of M r ∼ 10 000, that of the phosphate translocator ( M r 29 000) of M r ∼ 11 000 and that of M r 36 000 polypeptide of M r ∼ 2000 larger than the authentic protein.