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Rapid identification of Trypanosoma cruzi isolates by ‘dot‐spot’ hybridization
Author(s) -
Sanchez Daniel O.,
Madrid Raul,
Engel Juan C.,
Frasch Alberto C.C.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80223-9
Subject(s) - minicircle , trypanosoma cruzi , kinetoplast , biology , hybridization probe , kinetoplastida , dna–dna hybridization , dna , xenodiagnosis , identification (biology) , microbiology and biotechnology , parasite hosting , genetics , botany , world wide web , computer science
The presence of isolate‐specific Trypanosoma cruzi minicircles has been shown in the kinetoplast DNA of this parasite. This led to the rapid identification of isolates and clones of trypanosomes by means of ‘dot‐spot’ hybridizations with molecularly cloned minicircle probes. Unexpectedly, whole kDNAs were also suitable as probes for this purpose, provided that filters were washed under stringent conditions. This was attributed to the presence of the above‐mentioned isolate‐specific minicircle sequences. The fact that parasites could be directly spotted onto nitrocellulose filters simplified the rapid routine screening of a large number of samples.