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Calcium ions can be substituted for the 24‐kDa polypeptide in photosynthetic oxygen evolution
Author(s) -
Miyao Mitsue,
Murata Norio
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80218-5
Subject(s) - oxygen evolution , photosystem ii , photosynthesis , chemistry , oxygen , manganese , chloroplast , photosystem , spinach , sodium , photosystem i , calcium , magnesium , biophysics , biochemistry , photochemistry , biology , electrochemistry , organic chemistry , electrode , gene
Photosystem II particles were prepared from spinach chloroplasts with Triton X‐100, and treated with 1.0 M NaCl to remove polypeptides of 24 kDa and 18 kDa and to reduce the photosynthetic oxygen‐evolution activity by about half. Oxygen‐evolution activity was restored almost to the original level with 10 mM Ca 2+ , in a similar manner to the rebinding of 24‐kDa polypeptide. Other cations such as magnesium, sodium and manganese ions could not restore any oxygen‐evolution activity. These observations, together with a kinetic analysis, suggest that Ca 2+ can be substituted for the 24‐kDa polypeptide in photosynthetic oxygen evolution in Photosystem II particles.