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Cell cycle‐related changes in the surface properties of amoebae of the cellular slime mould Dictyostelium discoideum
Author(s) -
Sharpe Paul T.,
Watts D.J.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80212-4
Subject(s) - dictyostelium discoideum , slime mold , mycetozoa , axenic , microbiology and biotechnology , dictyostelium , biology , chemistry , biophysics , biochemistry , bacteria , genetics , gene
Amoebae of the cellular slime mould Dictyostelium discoideum were harvested during exponential, axenic growth and were partitioned in a dextran‐poly(ethylene glycol) two‐phase system in a countercurrent distribution apparatus. Amoebae in G1‐, S‐ and G2‐phases of the cell cycle were located in different parts of the countercurrent distribution. Since partitioning separates cells with different surface properties, it is concluded that there are cell cycle‐related changes in the surface properties, and thus plasma membrane structure, of the amoebae.