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Effects of FSH on acidic nuclear protein synthesis in cultured pig Sertoli cells
Author(s) -
Perrard M.H.,
Saez J.M.,
Dazord A.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80204-5
Subject(s) - sertoli cell , protein biosynthesis , nuclear protein , polyacrylamide gel electrophoresis , gel electrophoresis , chemistry , microbiology and biotechnology , biology , endocrinology , medicine , biochemistry , spermatogenesis , enzyme , gene , transcription factor
We studied the effect of FHS on nuclear protein synthesis by a primary culture of immature porcine Sertoli cells. The cells were cultured in a chemically defined medium and treated with FSH (50 ng/ml) for various periods (4, 8, 25 and 64 h). About 65 spots (pH i < 8) were identified by two‐dimensional polyacrylamide gel electrophoresis of the radiolabelled nuclear proteins. After 25 h of FHS treatment, we observed an increase of 5 proteins, a slight decrease of two and a disappearance of one. For the short periods of FSH treatment (4 and 8 h), no effect of FHS on nuclear protein synthesis was observed. After 64 h of FSH treatment, the synthesis of all nuclear proteins appears to be decreased.