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Specific leukotriene formation by purified human eosinophils and neutrophils
Author(s) -
Verhagen Jan,
Bruynzeel Pieter L.B.,
Koedam Johannes A.,
Aryan Wassink G.,
de Boer Martin,
Terpstra Gerben K.,
Kreukniet Johannes,
Veldink Gerrit A.,
Vliegenthart Johannes F.G.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80199-4
Subject(s) - granulocyte , leukotriene b4 , arachidonic acid , leukotriene , eosinophil , chemotaxis , leukotriene c4 , chemistry , calcium , biochemistry , neutrophile , incubation , metabolite , ionophore , basophil , immunology , biology , enzyme , inflammation , immunoglobulin e , in vitro , antibody , asthma , organic chemistry , receptor
Human granulocytes isolated from peripheral blood have been described to synthesize both LTB 4 and LTC 4 from arachidonic acid. We have observed that the amount of LTC 4 produced by human granulocyte preparations is strongly dependent on the relative amount of eosinophils. To investigate a possibly significant difference in leukotriene synthesis of the eosinophilic and neutrophilic granulocytes, we developed a purification method to isolate both cell types from granulocytes obtained from the blood of healthy donors. Leukotrienes were generated by incubation of the purified cells with arachidonic acid, calcium ionophore A23187, calcium‐chloride and reduced glutathione. Surprisingly, eosinophils were found to produce almost exclusively the spasmogenic LTC 4 . In contrast, neutrophils produce almost exclusively the chemotactic LTB 4 , its ω‐hydroxylated metabolite 20‐hydroxy‐LTB 4 and two non‐enzymically formed LTB 4 isomers.