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Analysis of brain metabolism changes induced by acute potassium cyanide intoxication by 31 P NMR in vivo using chronically implanted surface coils
Author(s) -
Decorps M.,
Lebas J.F.,
Leviel J.L.,
Confort S.,
Remy C.,
Benabid A.L.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80195-7
Subject(s) - phosphocreatine , cyanide , potassium , chemistry , in vivo , inorganic phosphate , phosphate , potassium cyanide , metabolism , energy metabolism , biochemistry , medicine , inorganic chemistry , biology , microbiology and biotechnology , organic chemistry
Chronic implantation of surface coils on the skull has been developed to record 31 P NMR spectra of the brain in unanesthetized rats. Intraperitoneal sublethal potassium cyanide doses induce strong and reversible changes in high‐energy phosphate compounds in the brain, similar in part to those induced by ischemia. These effects are dose‐dependent as far as phosphocreatine, inorganic orthophosphates and pH are concerned; ATP does not seem to be altered by KCN doses ranging from 3 to 5 mg/kg but starts decreasing at a dose of 6 mg/kg. The fraction of Mg 2+ complexed ATP which could be estimated as about 90% was not affected by KCN intoxication. For high doses (6 mg/kg) a new peak, appearing on the upfield side of the inorganic phosphate peak, may correspond to an acidic compartment, the significance of which is discussed.