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EPR titration of ovine prostaglandin H synthase with hemin
Author(s) -
Ruf H.H.,
Schuhn D.,
Nastainczyk W.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80189-1
Subject(s) - hemin , electron paramagnetic resonance , titration , chemistry , heme , atp synthase , enzyme , biochemistry , stereochemistry , inorganic chemistry , nuclear magnetic resonance , physics
To characterize further the prosthetic group of PGH synthase (EC 1.14.99.1), titrations of the apoenzyme with hemin were investigated by EPR. The first hemin bound per polypeptide showed an EPR signal at g = 6.7 and 5.3 (rhombicity 9%) and was tentatively assigned to the hemin effective as prosthetic group of PGH synthase. Additional hemin bound showed a less rhombic signal ( g = 6.3 and 5.8, rhombicity 3%) presumably due to nonspecific hydrophobic binding sites not effective in catalysis.