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A putative primer for the replication of cauliflower mosaic virus by reverse transcription is virion‐associated
Author(s) -
Turner David S.,
Covey Simon N.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80187-8
Subject(s) - cauliflower mosaic virus , reverse transcriptase , dna , biology , rna , transcription (linguistics) , retrovirus , microbiology and biotechnology , primer (cosmetics) , primase , virology , genetics , virus , chemistry , gene , genetically modified crops , linguistics , transgene , philosophy , organic chemistry
We have isolated, from a cauliflower mosaic virus (CaMV) preparation, an RNA species (75 nucleotides) covalently linked to the 5′‐end of the CaMV DNA fragment (sa‐DNA) which is complementary to the CaMV 35 S RNA transcript 5′‐end. Site‐specific cleavage located a unique m 7 G residue in the RNA moiety of sa‐DNA suggesting that it is host cell tRNA met i sa‐DNA is reminiscent of retrovirus ‘strong‐stop“ DNA and is evidence fo the reverse transcription model of CaMV replication.