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Fluorescence polarization study of tertiary structure of DNA within bacteriophage γ
Author(s) -
Shurdov M.A.,
Gruzdev A.D.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80176-3
Subject(s) - acridine orange , bacteriophage , dna , lambda , fluorescence , lambda phage , polarization (electrochemistry) , protein tertiary structure , chemistry , fluorescence anisotropy , biophysics , crystallography , microbiology and biotechnology , physics , biology , optics , biochemistry , escherichia coli , apoptosis , gene
The fluorescence polarization of acridine orange‐stained oriented γ phages was measured. The parameters of DNA packing within the phage head and were calculated (φ, angle between the direction of a small segment of DNA and the phage axis). It is shown that simple models of γ phage DNA tertiary structure are not consistent with calculated values. A new model is proposed.