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Stimulation of cyclic AMP in chondrocyte cultures: effects on sulfated‐proteoglycan synthesis
Author(s) -
Malemud Charles J.,
Papay Robert S.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80154-4
Subject(s) - ibmx , proteoglycan , intracellular , stimulation , biosynthesis , sulfation , incubation , chondrocyte , endocrinology , medicine , chemistry , biochemistry , cyclic adenosine monophosphate , biology , forskolin , in vitro , enzyme , receptor , extracellular matrix
The effects of N 6 , O 2 ′‐dibutyryladenosine 3′:5′‐cyclic monophosphate (DBcAMP), 8‐bromoadenosine 3′:5′‐cyclic monophosphate (8Br‐cAMP), 3′:5′‐cyclic monophosphate (cAMP), L‐isoproterenol and L‐epinephrine on sulfated‐proteoglycan synthesis by rabbit articular chondrocytes were compared. DBcAMP and 8Br‐cAMP in the presence or absence of 3‐isobutyl‐1‐methylxanthine (IBMX) stimulated sulfated‐proteoglycan biosynthesis after 20 h of incubation. cAMP had no significant effect. Both DBcAMP and 8Br‐cAMP increased by hydrodynamic size of the newly synthesized proteoglycan monomer (A1D1) relative to control cultures. By contrast, although isoproterenol and epinephrine stimulated total cAMP synthesis, neither stimulated sulfated‐proteoglycan synthesis. Whereas intracellular cAMP accumulated after incubation with DBcAMP and 8Br‐cAMP, this was not the case with isoproterenol whether IBMX was present or not. Thus, stimulation of sulfated‐proteoglycan synthesis by cAMP analogues in chondrocyte cultures appears to be dependent on increased intracellular cAMP accumulation rather than total cAMP biosynthesis.