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Nucleotide sequence of the Galleria mellonella nuclear polyhedrosis virus origin of DNA replication
Author(s) -
Blinov V.M.,
Gutorov V.V.,
Holodilov N.G.,
Iljichev A.A.,
Karginov V.A.,
Mikrjukov N.N.,
Mordvinov V.A.,
Nikonov I.V.,
Petrov N.A.,
Urmanov I.H.,
Vasilenko S.K.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80137-4
Subject(s) - galleria mellonella , plasmid , recombinant dna , nuclear polyhedrosis virus , biology , replicon , dna , virology , origin of replication , rolling circle replication , genome , virus , microbiology and biotechnology , dna replication , genetics , gene , virulence
The initiation sites of the Galleria mellonella L. nuclear polyhedrosis virus ( G.m. NPV) DNA replication were revealed. For this purpose SCLd 135 cells permitting the G.m. NPV productive reproduction were transformed by the recombinant plasmids containing the viral genome individual fragments in pRSF 2124 and pBR 322 vectors. It was revealed that 2 of the 32 recombinant plasmids can autonomously replicate in the eucaryotic cells. According to the Maxam‐Gilbert method the DNA G.m. NPV fragment (1300 bp) primary structure of pHBR plasmid was determined. The structure analysis revealed the typical regulator signals as in the replicons. The possible regulation mechanisms of the DNA G.m. NPV synthesis initiation was supposed.