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Phalloidin depletes the mitochondrial Ca 2+ compartment of hepatocytes
Author(s) -
Faulstich Heinz,
Münter Klaus,
Mayer D.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80134-9
Subject(s) - ionophore , phalloidin , mitochondrion , biophysics , incubation , chemistry , compartment (ship) , calcium , biochemistry , membrane , biology , cell , cytoskeleton , oceanography , organic chemistry , geology
In 3 h of incubation, primary cultures of rat hepatocytes attach to the substratum and exchange about 2.2 nmol 45 Ca 2+ per mg protein. In the presence of 1 μM phalloidin, the exchanged amount of 45 Ca 2+ was found to be decreased by about 30%. Using the uncoupling agent FCCP and the ionophore A23187 for further characterisation we determined that the 45 Ca 2+ deficit caused by phalloidin occurs in the FCCP‐sensitive compartment, i.e., the mitochondria.