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Reconstitution of solubilised brain D 2 dopamine receptors into phospholipid vesicles
Author(s) -
Wheatley M.,
Strange P.G.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80119-2
Subject(s) - vesicle , receptor , chemistry , phospholipid , sodium cholate , biochemistry , sodium , caudate nucleus , chromatography , biophysics , biology , organic chemistry , membrane , endocrinology
D 2 dopamine receptors have been solubilised from bovine caudate nucleus using cholate/sodium chloride in the presence of soyabean phospholipid. Reconstitution of the receptors into soyabean phospholipid vesicles has been achieved by dialysis to remove detergent and salt. The receptors are truly reconstituted as judged by sedimentation, electron microscopy, heat stability and analysis on sucrose density gradients. The ligand‐binding properties of the reconstituted receptors resemble those of the solubilised preparation.