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Isolation and amino acid sequence of a mating pheromone produced by mating type α cells of Saccharomyces kluyveri
Author(s) -
Sakurai Akira,
Sakamoto Mitsuhiro,
Tanaka Hiroko,
Esumi Yasuaki,
Takahashi Nobutaka,
Fujimura Hiroaki,
Yanagishima Naohiko,
Banno Isao
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80108-8
Subject(s) - pheromone , saccharomyces cerevisiae , mating , peptide sequence , biochemistry , mating type , amino acid , biology , peptide , sequence (biology) , enzyme , chemistry , stereochemistry , yeast , gene , genetics
A peptide, termed α sk2 pheromone, was isolated from culture filtrates of mating type α cells of Saccharomyces kluyveri as a mating pheromone, having both shmoo inducing (in a cells of S. kluyveri and S. cerevisiae ) and agglutinability inducing (in a cells of S. cerevisiae ) actions. The amino acid sequence of α sk2 pheromone was determined as H—Trp—His—Trp—Leu—Ser—Phe—Ser—Lys—Gly—Glu—Pro—Met—Tyr‐OH by mass spectrometry, sequence analysis and enzymatic digestions.