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An efficient method for labelling antibodies with 111 In
Author(s) -
Buckley R.G.,
Searle F.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80073-3
Subject(s) - conjugate , radioimmunoassay , antibody , labelling , monoclonal antibody , chemistry , size exclusion chromatography , conjugated system , chelation , filtration (mathematics) , chromatography , microbiology and biotechnology , biochemistry , immunology , biology , enzyme , organic chemistry , mathematics , mathematical analysis , statistics , polymer
Using a new method, rabbit IgG and a monoclonal antibody have been conjugated with the chelating agent DTPA. This was accomplished with reaction conditions that should entail lower antibody damage than existing methods. Gel filtration of the 111 In‐labelled antibody conjugate indicated minimal damage to the antibody and radioimmunoassay showed no significant change in its immunological activity.