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Increases in permeability of Escherichia coli outer membrane induced by polycations
Author(s) -
Katsu Takashi,
Yoshimura Shuichi,
Fujita Yuzaburo
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80067-8
Subject(s) - polylysine , efflux , chemistry , biophysics , membrane , permeability (electromagnetism) , escherichia coli , bacterial outer membrane , cytoplasm , cell membrane , biochemistry , biology , gene
The action of polycations (such as polylysine and compound 48/80) on Escherichia coli was studied with use of Ca 2+ , K + , and TPP + ion‐selective electrodes. Rapid efflux of Ca 2+ was observed when a polycation was added in cell suspension. The polycation treatment promoted a drug‐inducing K + release from the cytoplasmic membrane. TPP + uptake was also increased by addition of a polycation. Without the polycation treatment, the uptake of TPP + was largely suppressed due to a permeability barrier of the outer membrane. The results show that a polycation disrupted the permeability barrier of the outer membrane.