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A single subunit P‐700 reaction center of the thermophilic cyanobacterium Mastigocladus laminosus
Author(s) -
Muster Peter,
Binder Andres,
Bachofen Reinhard
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80064-2
Subject(s) - thermophile , photosynthetic reaction centre , protein subunit , photosystem ii , cyanobacteria , chemistry , photosystem i , biochemistry , photochemistry , cytochrome , enzyme , crystallography , photosynthesis , biology , bacteria , gene , genetics
The isolation and characterization of a single subunit Photosystem I reaction center of the thermophilic cyanobacterium Mastigocladus laminosus is described. The subunit isolated in 0.1% SDS is fully active in P‐700 bleaching and oxidizes cytochrome c in the light. Depending on denaturing conditions, the protein appears on SDS—PAGE in the 70 kDa or 50 kDa region and contains bound chlorophyll in both forms. The 70 kDa form shows all the activities of an intact reaction center.