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Photoaffinity labeling of acetylcholine receptor in millisecond time scale
Author(s) -
Muhn Peter,
Fahr Alfred,
Hucho Ferdinand
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80061-7
Subject(s) - millisecond , photoaffinity labeling , chemistry , biophysics , acetylcholine , receptor , neuroscience , biochemistry , pharmacology , biology , physics , astronomy
Photoaffinity labeling of acetylcholine receptors can be performed with a time resolution allowing to discriminate reaction sites within the receptor protein in its different functional states. This is achieved by a combination of a stopped‐flow apparatus with a high energy pulse laser. The photoaffinity label used is the lipophilic cation [ 3 H]TPMP + which has been shown to be a non‐competitive antagonist and a specific ion channel blocker. AChR in its resting (channel closed) and active (channel open) state incorporates the label mainly into the α‐polypeptide chain of the receptor. Only several hundred milliseconds after mixing AChR with agonist labeling of δ‐chains becomes significant.