Premium
Platelet aggregating activity of lysophosphatidic acids is not related to their calcium ionophore properties
Author(s) -
Simon Marie-Françoise,
Chap Hugues,
Douste-Blazy Louis
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80055-1
Subject(s) - ionophore , lysophosphatidic acid , calcium , chemistry , platelet , biophysics , vesicle , membrane , biochemistry , receptor , medicine , organic chemistry , biology
The calcium ionophore properties of A23187 and of two lysophosphatidic acid (LPA) analogs (1‐palmitoyl‐ and 1‐hexadecyl‐ sn ‐glycero‐3‐phosphate or P‐GPA and H‐GPA, respectively) were compared using platelet membrane vesicles loaded with 45 Ca. Half maximal effect (HME) was obtained at 5 μM and 10 μM for H‐GPA and P‐GPA, respectively, against 0.7 μM for A23187, which released 2 times more Ca. The three compounds also induced platelet aggregation with a HME at 0.5 μM, 0.3 μM and 0.01 μM for A23187, P‐GPA and H‐GPA, respectively. The clear dissociation between the two effects appearing for both LPA raises some doubt about the general idea that (lyso) PA participate in cell activation through their calcium ionophore properties.