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Expression of human α 1 ‐antitrypsin in Escherichia coli
Author(s) -
Bollen Alex,
Loriau Rosette,
Herzog Albert,
Hérion Pascal
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80046-0
Subject(s) - escherichia coli , promoter , recombinant dna , plasmid , repressor , microbiology and biotechnology , dna , transcription (linguistics) , biology , gene , expression vector , chemistry , genetics , transcription factor , gene expression , linguistics , philosophy
Complementary DNA coding for human α 1 ‐antitrypsin has been placed under the control of the λP R promotor carrier by the expression vector pCQV2 [1]. In conditions which allow transcription from this promotor (thermoinactivation of the repressor), Escherichia coli cells harbouring the recombinant plasmid pULB1114 express human α 1 ‐antitrypsin (± 9000 molecules/cell). The product has a M r of 44 000, corresponding to mature unglycosylated α 1 ‐antitrypsin.