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Effect of leukotriene B 4 , prostaglandin E 2 and arachidonic acid on cytosolic‐free calcium in human neutrophils
Author(s) -
Lew P.Daniel,
Dayer Jean-Michel,
Wollheim Claes B.,
Pozzan Tullio
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80041-1
Subject(s) - leukotriene b4 , arachidonic acid , extracellular , cytosol , chemistry , chemotaxis , calcium , leukotriene , biochemistry , intracellular , leukotriene c4 , lipoxygenase , enzyme , biology , inflammation , immunology , receptor , organic chemistry , asthma
Changes in cytosolic free calcium [Ca 2+ ] i and release of beta‐glucuronidase in response to leukotriene B 4 (LTB 4 ) were measured in intact neutrophils loaded with the fluorescent Ca 2+ indicator, quin 2. LTB 4 (10 −10 M or higher) caused a rapid rise in [Ca 2+ ] i due to influx from the extracellular medium and release from intracellular pools as well as enzyme release. PGE 2 (3 μM) did not alter [Ca 2+ ] i whereas arachidonic acid (10 μM) raised [Ca 2+ ] i . Pretreatment of cells with the chemotactic peptide FMLP inhibited the subsequent rise of [Ca 2+ ] i induced by LTB 4 . Since chemotactic peptides activate the lipoxygenase pathway of arachidonic acid metabolism, it may be speculated that endogenous LTB 4 generation is involved in neutrophil activation.