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Protein synthesis by isolated chloroplasts of Chlamydomonas reinhardii
Author(s) -
Leu Stefan,
Mendiola-Morgenthaler Leticia,
Boschetti Arminio
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80037-x
Subject(s) - chlamydomonas , chloroplast , protein biosynthesis , biochemistry , protein subunit , biology , amino acid , pyrenoid , mutant , gene
Chloroplasts which have been isolated from the cell wall mutant cw15‐2 of Chlamydomonas reinhardii are active in light‐dependent, in organello protein synthesis. Certain characteristics of protein synthesis in this system were tested, such as time course of amino acid incorporation, sensitivities to Mg 2+ as well as to specific inhibitors, and found to be typical of chloroplasts isolated from higher plants. The major products made were identified as the large subunit of ribulose‐1,5‐biphosphate carboxylase and the 32‐kDa rapidly labelled membrane protein.