Premium
Effects of proteolysis on the actions of monovalent cations and 1‐ O ‐octadecyl‐2‐ O ‐acetyl‐ sn ‐glyceryl‐3‐phosphorylcholine on platelet adenylate cyclase
Author(s) -
Williams Kimberley A.,
Vanderwel Marianne,
Haslam Richard J.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80035-6
Subject(s) - chemistry , proteolysis , cyclase , adenylate kinase , platelet , phosphorylcholine , biochemistry , enzyme , medicinal chemistry , stereochemistry , biology , immunology
NaCl stimulated the adenylate cyclase activities of human and rabbit platelet particulate fractions prepared in the presence of ethylene glycol bis(β‐aminoethyl ether)‐ N,N,N ′ ,N ′‐tetraacetate, but inhibited the activities of particulate fractions proteolysed by endogenous Ca 2+ ‐activated protease or treatment with α‐chymotrypsin. Studies with other monovalent cations showed that LiCl had weak effects similar to those of NaCl, whereas KCl inhibited the enzyme in both proteolysed and non‐proteolysed preparations. The results suggest that NaCl exerts stimulatory and inhibitory effects through different sites. NaCl potentiated and proteolysis greatly reduced the inhibition of platelet adenylate cyclase by 1‐ O ‐octadecyl‐2‐ O ‐acetyl‐ sn ‐glyceryl‐3‐phosphorylcholine (platelet‐activating factor).