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Axonal transport of muscarinic receptors in vesicles containing noradrenaline and dopamine‐β‐hydroxylase
Author(s) -
Laduron Pierre M.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80028-9
Subject(s) - muscarinic acetylcholine receptor , dopamine , quinuclidinyl benzilate , chemistry , neurotransmitter , endocrinology , medicine , receptor , biology , biochemistry
Presynaptic muscarinic receptors labeled with [ 3 H]dexetimide and noradrenaline in dog splenic nerves accumulated proximally to a ligature at the same rate of axonal transport. After fractionation by differential centrifugation, specific [ 3 H]quinuclidinyl benzilate or [ 3 H]dexetimide binding revealed a distribution profile similar to that of dopamine‐β‐hydroxylase and noradrenaline. Subfractionation by density gradient centrifugation showed two peaks of muscarinic receptors; the peak of density 1.17 contained noradrenaline and dopamine‐β‐hydroxylase whereas that of density 1.14 was devoid of noradrenaline. Therefore the foregoing experiments provide evidence that presynaptic muscarinic receptors are transported in sympathetic nerves in synaptic vesicles which are similar to those containing noradrenaline and dopamine‐β‐hydroxylase. This suggests a possible coexistence of receptor and neurotransmitter in the same vesicle.