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Critical role of sulfhydryl group(s) in ATP‐dependent Ca 2+ sequestration by the plasma membrane fraction from rat liver
Author(s) -
Bellomo Giorgio,
Mirabelli Francesca,
Richelmi Plinio,
Orrenius Sten
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)81180-6
Subject(s) - dithiothreitol , chemistry , glutathione , biochemistry , membrane , oxidative stress , oxidizing agent , homeostasis , oxidative phosphorylation , biophysics , enzyme , endocrinology , biology , organic chemistry
The ATP‐dependent sequestration of Ca 2+ by the plasma membrane fraction from rat liver is stimulated by reduced glutathione and dithiothreitol and inhibited by diamide and t ‐butyl hydroperoxide. The inhibitory effect on Ca 2+ sequestration by the oxidizing agents is prevented in the presence of the thiols. Our results therefore suggest that free sulfhydryl group(s) may be critical for the activity of hepatic plasma membrane Ca 2+ translocase, and that inhibition of this activity by the oxidation of such group(s) may contribute to the perturbation of Ca 2+ homeostasis during oxidative stress.