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The fluorescence response of chlortetracycline‐loaded human neutrophils is modulated by prostaglandin E 1 , but not by cyclic nucleotides
Author(s) -
Smolen James E.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)81176-4
Subject(s) - nucleotide , chlortetracycline , chemistry , fluorescence , prostaglandin , cyclic nucleotide , prostaglandin e , microbiology and biotechnology , biochemistry , antibiotics , biology , physics , optics , gene
Human neutrophils preloaded with chlortetracycline, commonly used as a probe of membrane‐bound calcium, demonstrate a prompt decrease in fluorescence when exposed to surface stimuli such as the chemotactic peptide fMet—Leu—Phe. The fluorescence response was highly sensitive to preincubation with prostaglandin E 1 . This effect was apparently not due to elevated levels of cAMP since exogenous dibutyryl‐cAMP did not alter the chlortetracycline fluorescence response to fMet—Leu—Phe. This is one of the few instances of prostaglandin E 1 affecting neutrophils at physiologic concentrations, dissociated from changes in cellular cyclic nucleotide levels