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Potentiating effect of heparin in the activation of procollagenase by a low‐ M r angiogenesis factor
Author(s) -
Weiss Jacqueline B.,
Hill C.R.,
McLaughlin B.,
Elstow S.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)81163-6
Subject(s) - angiogenesis , heparin , trypsin , chemistry , fibroblast , mechanism of action , fibroblast growth factor , endothelial stem cell , cell culture , cell , microbiology and biotechnology , biochemistry , biophysics , cancer research , biology , receptor , in vitro , enzyme , genetics
A low‐ M r freely dialysable endothelial cell‐stimulating angiogenesis factor (ESAF) from conditioned medium of a mouse lymphoma cell line has previously been shown to activate latent skin fibroblast procollagenase. Activation comparable with the maximum that can be achieved with trypsin is obtained with chemically undetectable amounts of the factor. We now show that when even smaller amounts of ESAF are used heparin is able to potentiate its action in this system. The relationship between this activity and the mechanism of angiogenesis, which is itself potentiated by heparin, is discussed.