Premium
Electrofusion of myeloma cells on the single cell level
Author(s) -
Vienken J.,
Zimmermann U.,
Fouchard M.,
Zagury D.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)81161-2
Subject(s) - electrofusion , petri dish , pronase , fusion , cell fusion , chemistry , materials science , chromatography , biophysics , cell , biology , biochemistry , composite material , trypsin , microbiology and biotechnology , linguistics , philosophy , enzyme
A technique is presented which allows electrofusion of single cells under sterile conditions. The electrofusion chamber is placed in a Petri dish. Before a droplet of the fusion medium is pipetted between the electrodes, the chamber is completely covered with vaseline, which prevents the fusion medium evaporating. Additionally, the fusion chamber is treated with solutions containing poly(L)‐lysine and pronase which results in a decreased movement of the cells on the glass between the electrodes and which allows electrofusion without any proteolytic pretreatment.