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Resolution of the hemes of hydroxylamine oxidoreductase by redox potentiometry and optical spectroscopy
Author(s) -
Prince Roger C.,
Larroque Christian,
Hooper Alan B.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)81154-5
Subject(s) - hydroxylamine , chemistry , chromophore , redox , heme , spectroscopy , resolution (logic) , oxidoreductase , stoichiometry , heme a , photochemistry , analytical chemistry (journal) , inorganic chemistry , organic chemistry , physics , quantum mechanics , artificial intelligence , computer science , enzyme
Optical spectroscopy combined with redox potentiometry has resolved the hemes of hydroxylamine oxidoreductase into 6 thermodynamically distinct classes. There are apparently 4 classes of heme c 553 , with E m7 ‐values of 295 mV, 10 mV, −190 mV and −390 mV, present in a stoichiometry of 1:1:2:1; two equivalents of heme c 559 , E m7 O mV, and one of heme P‐460, an unusual chromophore, with E m7 −260 mV.