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Inhibition of protein synthesis by the cordycepin analog of (2′–5′)ppp(Ap) n A, (2′–5′)ppp(3′dAp) n 3′ dA, in intact mammalian cells
Author(s) -
Lee Choongeun,
Suhadolnik Robert J.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)81146-6
Subject(s) - cordycepin , incubation , protein biosynthesis , microbiology and biotechnology , chemistry , stereochemistry , cytotoxic t cell , biochemistry , biology , in vitro
The introduction of the cordycepin analog of (2′–5′)A n , (2′–5′)ppp(3′ dAp) n 3′ dA [referred to as (2′–5′)p 3 3′ dA n ], into mouse L929 cells and cultured human fibroblasts resulted in a dose‐dependent inhibition of protein synthesis which was comparable to the inhibition observed by (2′–5′)ppp(Ap) n A [referred to as (2′–5′)p 3 A n ]. The inhibition of protein synthesis by (2′–5′)p 3 3′ dA n was much more persistent than that of the naturally occurring (2′–5′)p 3 A n following prolonged incubation of cells. Furthermore, the (2′–5′)p 3 A n was cytotoxic to mammalian cells in culture, whereas the (2′–5′)p 3 3′dA n was not.