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Phosphatidylserine decarboxylase is located on the external side of the inner mitochondrial membrane
Author(s) -
Zborowski Józef,
Dygas Anna,
Wojtczak Lech
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)81141-7
Subject(s) - phosphatidylserine , inner membrane , inner mitochondrial membrane , trypsin , succinate dehydrogenase , mitochondrion , cytochrome c oxidase , bacterial outer membrane , biochemistry , chemistry , membrane , enzyme , microbiology and biotechnology , biology , phospholipid , escherichia coli , gene
It is shown that the trypsin‐treatment of rat liver mitochondria, depleted of the outer membrane, causes a strong inactivation of phosphatidylserine decarboxylase. This inactivation is dependent on trypsin concentration and the time of digestion in a similar manner as the inactivation of cytochrome oxidase. Under these conditions only a moderate inactivation of succinate dehydrogenase is observed. Phosphatidylserine decarboxylase is thus localized in the outer leaflet of the inner mitochondria membrane or, at least, is accessible from the outer surface of the inner membrane.