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Preferential utilization of endogenous arachidonate by cyclo‐oxygenase in incubations of human platelets
Author(s) -
Sautebin L.,
Caruso D.,
Galli G,
Paoletti R.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)81140-5
Subject(s) - platelet , endogeny , chemistry , oxygenase , biochemistry , enzyme , biology , immunology
Thromboxane B 2 (TXB 2 ) and 12‐hydroxy‐5,8,10,14‐eicosatetraenoic acid (12‐HETE) formed from the endogenous and exogenous arachidonate during human platelet incubation, was evaluated by selected ion monitoring (SIM). TXB 2 formed from endogenous substrate accounted for about one third of the total, whereas the great part of 12‐HETE derived from exogenous arachidonate. These data indicate that under the tested conditions the pool of arachidonate that acts as substrate for cyclo‐oxygenase is different from the pool that acts as substrate for lipoxygenase and that the arachidonate released from phospholipids is preferentially utilized by cyclo‐oxygenase.