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Interaction of nucleic acids with the DNA‐dependent RNA polymerases of Drosophila
Author(s) -
Gundelfinger Eckart D.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)81131-4
Subject(s) - polymerase , nucleic acid , rna , dna , biochemistry , rna dependent rna polymerase , rna polymerase , dna polymerase , microbiology and biotechnology , chemistry , biology , gene
The interaction between the three Drosophila DNA‐dependent RNA polymerases (EC 2.7.7.6) and the DNA template or the RNA product was investigated by photochemical cross‐linking and binding studies, using RNA polymerase subunits immobilized on nitro‐cellulose filters. It can be shown that the two largest subunits are responsible for the binding of the enzymes to both template and newly‐synthesized RNA.