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Rapid purification of bovine kidney branched‐chain 2‐oxoacid dehydrogenase complex containing endogenous kinase activity
Author(s) -
Lawson Rowena,
Cook Kenneth G.,
Yeaman Stephen J.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)81115-6
Subject(s) - biochemistry , endogeny , phosphorylation , chemistry , dehydrogenase , enzyme , kinase
Branched‐chain 2‐oxoacid dehydrogenase complex has been purified to near homogeneity by a simple, rapid procedure. The final product contains endogenous kinase activity capable of phosphorylating and inactivating the complex. Phosphorylation continues after complete inactivation, indicating the possibility of several phosphorylatable sites.

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