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A rapid stimulation of phosphatidylinositol metabolism in rabbit leukocytes by pseudomonal leukocidin
Author(s) -
Hirayama Toshiya,
Kato Iwao
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)81113-2
Subject(s) - microbiology and biotechnology , stimulation , phosphatidylinositol , virology , chemistry , biology , biochemistry , signal transduction , neuroscience
The time course experiments of 32 P i ‐labelling and breakdown of phospholipids in rabbit leukocytes exposed to leukocidin from Pseudomonas aeruginosa suggested that the initial action of this toxin was to stimulate phosphatidic acid production, presumably by causing a rapid metabolic change of phosphatidylinositol (PI response) correlating with phosphatidylinositol‐specific phospholipase C and 1,2‐diacylglycerol kinase. It appears that a rapid formation of phosphatidic acid and degradation of polyphosphoinositides in leukocytes treated with the toxin might be related a Ca 2+ ‐movement from extra‐ and intracellular spaces, resuling in the activation of Ca 2+ ‐dependent enzymes involved in the leukocidic process.