Premium
Effect of pH on the photoreaction cycles of bacteriorhodopsin
Author(s) -
Kobayashi Takayoshi,
Ohtani Hiroyuki,
Iwai Jin-ichi,
Ikegami Akira,
Uchiki Hisao
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)81078-3
Subject(s) - bacteriorhodopsin , picosecond , suspension (topology) , nanosecond , chemistry , photochemistry , proton , spectral line , optics , biochemistry , membrane , mathematics , quantum mechanics , homotopy , pure mathematics , laser , physics , astronomy
Photoreaction cycles of acidified bacteriorhodopsin (bR acid 605 ) at room temperature was studied by nanosecond and picosecond spectroscopy. A precursor of bathobacteriorhodopsin was found to be converted to bathobacteriorhodopsin within 30 ps. Spectra and formation times of batho‐ and lumibacteriorhodopsin are identical to those of bacteriorhodopsin in neutral suspension. However, lumibacteriorhodopsin lifetime is > 700 μs and the formation of metabacteriorhodopsin was not observed in low pH suspension. Thus, the decay process of lumibacteriorhodopsin plays an important role in the proton pump action.