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Polarized absorption picosecond kinetics as a probe of energy transfer in phycobilisomes of Synechococcus 6301
Author(s) -
Gillbro Tomas,
Sandström Åke,
Sundström Villy,
Holzwarth Alfred R.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)81050-3
Subject(s) - phycobilisome , chromophore , allophycocyanin , picosecond , photochemistry , kinetics , chemistry , fluorescence anisotropy , fluorescence , molecular physics , phycocyanin , chemical physics , energy transfer , anisotropy , physics , optics , biology , cyanobacteria , laser , genetics , quantum mechanics , bacteria
The energy transfer between C‐phycocyanin chromophores in intact phycobilisomes of Synechococcus 6301 is shown to lead to an anisotropy relaxation with a lifetime of 10 ± 2 ps. However, due to the molecular order within the hexameric units of C‐phycocyanin the anisotropy does not decay to zero. The Förster dipole‐dipole mechanism of energy transfer can qualitatively explain these data provided that there is no back transfer of excitation energy and that the chromophore distribution is non‐random. The rate of energy transfer in phycobilisomes between C‐phycocyanin and allophycocyanin can best be described by a double exponential with lifetimes of 12 ± 3 and 84 ± 8 ps.