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The translation of the messenger for the poly(A)‐binding protein‐associated with translated mRNA is suppressed
Author(s) -
Akhayat Omar,
Vincent Alain,
Goldenberg Samuel,
Person Anne,
Scherrer Klaus
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)81042-4
Subject(s) - messenger rna , translation (biology) , protein biosynthesis , in vivo , in vitro , cytoplasm , microbiology and biotechnology , biology , chemistry , biochemistry , gene , genetics
In vivo protein synthesis in duck erythroblasts was compared to in vitro translation of polyribosomal and free cytoplasmic mRNA. The in vivo study showed the absence of de novo synthesis of the M r 73 000 poly(A)‐binding protein found associated with all polyribosomal mRNA. In vitro translation demonstrated that the mRNA for this protein is absent from the polyribosomal mRNA fraction but constitutes a medium frequency messenger among the repressed free mRNA. This result confirms the existence of a qualitative translational control in terminal differentiating duck erythroblasts leading eventually to the arrest of the protein synthesizing machinery.