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Does the lactose carrier of Escherichia coli function as a monomer?
Author(s) -
Keith Wright J.,
Weigel Ulrich,
Lustig Ariel,
Bocklage Hubertus,
Mieschendahl Martin,
Müller-Hill Benno,
Overath Peter
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)81039-4
Subject(s) - monomer , lactose permease , galactoside , escherichia coli , mutant , lactose , chemistry , symporter , beta galactosidase , micelle , lac operon , biochemistry , permease , polymer , transporter , gene , organic chemistry , enzyme , aqueous solution
The purified lactose carrier of Escherichia coli (product of the lacY gene) is shown to be a monomer in detergent micelles of dodecyl‐ O ‐β‐D‐maltoside. The negative‐dominant phenotype of mutant carriers ( lacY −d mutants) could not be verified by measurements of the rate of galactoside transport in lacY + / Y −d diploid strains. It is proposed that the membrane‐embedded carrier functions as a monomer in galactoside‐H + symport.