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Messenger RNA activities of four acute phase proteins during inflammation
Author(s) -
Northemann Wolfgang,
Andus Tilo,
Gross Volker,
Nagashima Mariko,
Schreiber Gerhard,
Heinrich Peter C.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)81033-3
Subject(s) - acute phase protein , transferrin , messenger rna , orosomucoid , albumin , hemopexin , glycoprotein , alpha (finance) , inflammation , sialic acid , translation (biology) , rna , alpha 2 macroglobulin , macroglobulin , microbiology and biotechnology , biochemistry , chemistry , biology , immunology , enzyme , medicine , heme , gene , construct validity , nursing , patient satisfaction
Poly(A) + RNA isolated from the livers of normal rats and of rats suffering from an acute inflammation was translated in a cell‐free translation system from rabbit reticulocytes. The translation products were immunoprecipitated with specific antisera against α1‐acid glycoprotein, α2‐macroglobulin, transferrin, α1‐proteinase inhibitor and albumin. 15 to 21 h after intramuscular injection of turpentine 73‐, 66‐, 2.8‐, and 2‐fold increases in translatable mRNAs for α1‐acid glycoprotein, α2‐macroglobulin, transferrin and α1‐proteinase inhibitor, respectively, were observed. For albumin a decrease in translatable mRNA to about 30% of controls was measured.