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Desaturation of oleoyl and linoleoyl residues linked to phospholipids in growing roots of yellow lupin
Author(s) -
Citharel B.,
Oursel A.,
Mazliak P.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)81019-9
Subject(s) - phosphatidylethanolamine , phosphatidylcholine , polyunsaturated fatty acid , chemistry , phospholipid , biochemistry , fatty acid , membrane
When growing lupin roots were fed with [ 14 C]oleate and [ 14 C]linoleate most of the absorbed molecules remained in the free fatty acid pool. Part of the 14 C‐labelled fatty acid was integrated first into phosphatidylcholine and subsequently into phosphatidylethanolamine. Then, progressive desaturation took place. The kinetics of these processes suggests that desaturation in phosphatidylcholine was followed by transacylation of polyunsaturated fatty acids into phosphatidylethanolamine. To produce good yields of desaturation (about 48%), seeds had to be less than 1‐year old. With respect to linoleate desaturation, seedlings had to be 2–7‐days old. Increased percentages of oleate desaturation were obtained when the roots were grown on a medium enriched with calcium.