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Magnesium as a natural substitute for manganese in concanavalin A and other lectins
Author(s) -
Martin Young N.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)81018-7
Subject(s) - manganese , concanavalin a , magnesium , chemistry , natural (archaeology) , biochemistry , environmental chemistry , biology , organic chemistry , in vitro , paleontology
Addition of magnesium to apo‐concanavalin A in the presence of calcium was shown by ultraviolet difference spectroscopy to generate a holoprotein spectroscopically identical to the MnCa‐holoprotein. The MgCa‐ and MnCa‐forms bound equally strongly to Sephadex G‐75. In kinetic experiments, the binding of Mg 2+ was much slower than Mn 2+ binding; K d for Mg 2+ was estimated as 7.4 mM. The combined Mg 2+ and Mn 2+ contents of 10 lectins specific for D‐galactose or N ‐acetyl‐D‐galactosamine were each close to one atom per subunit, suggesting occupancy of the Mn 2+ site by Mg 2+ is common in plant lectins.