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A new site‐specific endodeoxyribonuclease from Citrobacter freundii
Author(s) -
Janulaitis A.,
Stakenas P.,
Berlin Yu.A.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)81009-6
Subject(s) - citrobacter freundii , cloning (programming) , endonuclease , molecular cloning , chemistry , strain (injury) , dna , enzyme , computational biology , biochemistry , biology , microbiology and biotechnology , genetics , escherichia coli , enterobacteriaceae , peptide sequence , gene , computer science , programming language , anatomy
Cfr 10 I, a site‐specific endonuclease from Citrobacter freundii strain RFL 10, was isolated. It recognizes and cleaves the family of related sequences: 5'Pu ↓ CCGGPy to generate DNA fragments with 5' tetranucleotide extensions. Cfr 10 I may be useful in molecular cloning experiments, especially in conjunction with other enzymes which generate the same terminal extensions.